Software plugins for automatic cell counting in the Drosophila central nervous system

Forero, Manuel Guillermo and Hidalgo, Alicia (2025) Software plugins for automatic cell counting in the Drosophila central nervous system.

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Dataset Details

Data creator(s):	Creators Email ORCID Forero, Manuel Guillermo manuel.forero@ibague.edu.co orcid.org/0000-0001-9972-8621 Hidalgo, Alicia a.hidalgo@bham.ac.uk orcid.org/0000-0001-8041-5764
Research Data Type:	Software
DOI:	https://doi.org/10.25500/edata.bham.00001213
Publisher:	University of Birmingham
Funder:	Wellcome Trust
Keywords:	Drosophila; adult brain; cell counting; larval; cell number; synapse; central nervous system; NMJ; adult brain; larva; embryo; nuclei; neurons; glia; apoptotic cells; Caspase-3; Dcp1; HB9; Brp; Repo; Histone-YFP; pHistone-H3; pH3
Managing organisational unit:	Colleges (2008 onwards) > College of Life & Environmental Sciences
UoB School, Department or Institute:	School of Biosciences
Date:	13 January 2025

Additional Details

Additional Details

Project/Grant:	223197/Z/21/Z
Primary language:	English
Collection period:	From To 2004 2024
Data collection method:	Laser scanning confocal microscopy data used to generate all of the DeadEasy plugins were acquired from 2004 onwards until 2024, and the microscopy data were contributed by many different members of the Hidalgo Lab over this period.
Data processing and preparation activities:	Laser scanning confocal microscopy data were acquired from Drosophila fixed embryos and fixed and dissected larvae and adult brains. Cells were labelled either with antibodies or nuclear reporters (eg UAS-HistoneYFP). The development of the plugins involved multiple rounds of optimisation (details of this have been published already).
Depositing User:	Alicia Hidalgo
Date Deposited:	13 Jan 2025 16:16
Last Modified:	14 Jan 2025 11:57

Available Files

Data

DeadEasy_GliaAdult.ijm.txt DeadEasy_GliaAdult.ijm.txt DeadEasy_GliaAdult.ijm.txt Access option: Open File: DeadEasy_GliaAdult.ijm.txt Content type: Data Metadata Revision: 4 Mime-Type: text/plain Licence: File size: 1kB File checksum: e46cf2c3ac24689d771ea42a939ff618

DeadEasy ADULT ... enyon Cells.zip DeadEasy ADULT ... enyon Cells.zip DeadEasy ADULT ... enyon Cells.zip Access option: Open File: DeadEasy ADULT Kenyon Cells.zip Content type: Data Metadata Revision: 5 Mime-Type: application/zip Licence: File size: 17kB File checksum: c9fc7135c1b00c8481f317849729949b

FULL DEADEASY S ... %26 larvae.zip FULL DEADEASY S ... %26 larvae.zip FULL DEADEASY S ... %26 larvae.zip Access option: Open File: FULL DEADEASY SET embryo & larvae.zip Content type: Data Metadata Revision: 6 Mime-Type: application/zip Licence: File size: 217kB File checksum: b42ec8093c2316fd5817507fcf1b5617

DEADEASY OPTIC LOBE.zip DEADEASY OPTIC LOBE.zip DEADEASY OPTIC LOBE.zip Access option: Open File: DEADEASY OPTIC LOBE.zip Content type: Data Metadata Revision: 7 Mime-Type: application/zip Licence: File size: 4kB File checksum: f00aa2360ec9e5aa7dbaad2e6b6fbcc0

Cells_filter_Fo ... ro-Hidalgo.java Cells_filter_Fo ... ro-Hidalgo.java Cells_filter_Fo ... ro-Hidalgo.java Access option: Open File: Cells_filter_Forero-Hidalgo.java Content type: Data Metadata Revision: 8 Mime-Type: text/plain Licence: File size: 8kB File checksum: dbf63a293b16177f1f0829c813c0166d

DeadEasy_Dopami ... ero_Hidalgo.ijm DeadEasy_Dopami ... ero_Hidalgo.ijm DeadEasy_Dopami ... ero_Hidalgo.ijm Access option: Open File: DeadEasy_Dopaminergic3DNew_Forero_Hidalgo.ijm Content type: Data Metadata Revision: 9 Mime-Type: text/plain Licence: File size: 3kB File checksum: 569eb08b66f181f8aaa3761e5b482317

DeadEasy_Centra ... ero_Hidalgo.ijm DeadEasy_Centra ... ero_Hidalgo.ijm DeadEasy_Centra ... ero_Hidalgo.ijm Access option: Open File: DeadEasy_Central_Brain_Forero_Hidalgo.ijm Content type: Data Metadata Revision: 10 Mime-Type: text/plain Licence: File size: 2kB File checksum: 7c5d0f2283100a93f299dc54f8ff29a7

Read me

README1213-Instructions.txt README1213-Instructions.txt README1213-Instructions.txt Access option: Open File: README1213-Instructions.txt Content type: ["content_typename_readme" not defined] Metadata Revision: 4 Mime-Type: text/plain Licence: File size: 3kB File checksum: 379429f9df306a29e4d42123e5084e2b

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